Medium optimization for extracellular urate oxidase production by a newly isolated Aspergillus Niger

Urate oxidase is a peroxisomal enzyme with four equal subunits that convert uric acid to allantoin, more soluble metabolite for excretion. The usage of uricase as drug in medicine treat hyperuricemia. Many microorganisms have been used production such Streptomyces exfoliates, Pseudomonas aeruginosa, and Aspergillus flavus. In this study, soil samples were collected then cultured screening medium including the sole carbon source. Samples higher ability selected assay. Enzyme activity was measured by spectrophotometry sample maximal identified niger further studies. According results experiments, optimized temperature determined be 35±2°C. best nitrogen source glucose NH4NO3, highest observed presence Cu2+ ion.